Most residues in the active sites of CDK2, CDK4 and CDK6 are remarkably conserved (Figure 2). A key difference is the presence of a histidine residue in CDK4/6 (His95CDK4 and His100CDK6) while CDK2 comprises a phenylalanine (Phe82) in the equivalent position. The His95CDK4/His100CDK6 side-chain is in a position where it potentially can donate or accept […]

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Figure 4. Confirmation of anti-HCV activity for top 17 hits. A. Potency (EC50) against the gt 1a/2a-Rluc virus (EC50) in a 96 h multi-cycle assay and corresponding Huh-7.5 cell cytotoxicity (CC50) for each of the top 17 screen hits. B. Confirmation of anti-HCV activity through comparison of potency using Renilla Luciferase or HCV Core Cellomics […]

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screening formats using cell protection as a readout [19]. In a envelope glycoproteins (E1�E2). They had no activity against VSVpp pseudo-particles. Since the only HCV proteins contained in HCVpp are the envelope glycoproteins, these results suggest that the inhibitors target the viral proteins E1 and/or E2, or their functions are distinct from those involved in […]

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����Estrogen hormone carries out its physiological functionsthrough its receptors. ESRa is expressed by many of the trigeminalneurons. The gene encoding estrogen receptor alpha, ��ESR1��localized in 6q25.1 locus is the most widely studied hormonalpathway gene in migraine. We analyzed two intronic ��intron 1��and two exonic ��exons 4 & 8�� polymorphisms of this gene. ����However, we could […]

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