Ed in oMSCs treated with yEVs. With respect to pluripotency markers at RNA level, a statistically substantial lower of pluripotency markers (Nanog and Oct4) in yMSCs treated with oEVs in addition to a statistically substantial boost in oMSCs treated with yEVs had been obtained. On the contrary, it was obtained with respect Vinculin expression. At proteomic level, a statistically considerable increase of 3 isoforms of Lamin A/C in yMSCs treated with oEVs and statistically considerable decrease in oMSCs treated with yEVs had been obtained. Summary/conclusion: MSC-derived EVs and their self-renewal capacity were influenced by age, as reveled by modifications inside the expression of self-renewal markers like Nanog, Oct4, Vinculin and Lamin A/C. These findings are critical towards the fully grasp the influence with the ageing on MSCs and to advance in the improvement Caspase 13 Proteins Biological Activity EV-based therapies.PT06.Ex vivo expansion of umbilical cord blood-derived haematopoietic stem cells using extracellular vesicles of bone marrow stromal origin Corina Ghebes1; Jess Morhayim2; Marion Kleijer1; Bram van der Eerden3; Jeroen van de Peppel3; Eric Braakman2; Carlijn Voermans1 Division of Hematopoiesis, Sanquin Investigation, Amsterdam, The Netherlands; 2Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; 3Department of Internal Medicine, Erasmus University Healthcare Center, Rotterdam, The NetherlandsPT06.Functionality of bone marrow mesenchymal stem cell-derived extracellular vesicles on self-renewal Juan Antonio Fafian Labora1; Miriam Morente-L ez2; Patricia D zBarreiro2; Pablo Fernandez-Pernas2; Mar Arufe Gonda1 Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Glucocorticoid Receptor Proteins Storage & Stability Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade 1Grupo de Terapia Celular y Medicina Regenerativa (TCMRCHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina y Fisioterapia, Facultade de Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain. Oza, Universidade da Coru (UDC), As Xubias, A Coru , Spain., A Coru , Spain; 2Grupo de Terapia Celular y Medicina Regenerativa (TCMR-CHUAC), CIBER BBN/ISCIII, Instituto de Investigaci Biom ica de A Coru (INIBIC), Complexo Hospitalario Universitario de A Coru (CHUAC), SERGAS, Departamento de Ciencias Biom icas, Medicina, A Coru , SpainBackground: Mesenchymal stem cells (MSCs) are very relevant for regeneration of mesoderm tissues. The promising role of MSCs in cellbased therapies and tissue engineering is restricted due to a decline pluripotency, proliferation and immunogenic possible with growing donor age. Extracellular vesicles (EVs) have already been recognizing as potent cars of intercellular communication on diverse biological systems. MSC-derived EVs present positive aspects over cell-based therapy as it eliminates the safety issues linked with injection of MSCs in sufferers and specifically valuable for enhancing recovery from different illnesses. Right here, the influence of ageing on self-renewal functionality MSC-derived EVs utilizing an in vitro model was studied. Strategies: Two age groups from bone marrow MSCs of male Wistar rats had been applied, old (three months old) and young (14 days old). EVs wereBackground: Ex vivo expansion of umbilical cord blood (UCB)-deri.
