Product Name :
Shc monoclonal antibody (11F6)
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Stable at -80°C up to 1 year, at 4°C up to 3 months.
Description:
The Src homology 2 domain containing (Shc) adaptor protein links growth factor, cytokine, and antigen receptor stimulation to the Ras signaling pathway, and plays a critical role in insulin signaling, tumorigenesis, and hematopoietic cell growth and differentiation. Structurally, Shc is composed of an N-terminal phosphotyrosine binding (PTB) domain, a medial CH1 domain, and a C-terminal SH2 domain. Upon binding to activate cell-surface receptors, Shc is phosphorylated at three tyrosine residues (Tyr239, Tyr240, Tyr317) triggering recognition of Shc by SH2-containing proteins such as Grb2. Immunohistochemistry analysis of human hepatoma tissue with Shc mAb (11F6). Immunofluorescent analysis (confocal) staining of V79 cells using Shc mAb (11F6) (green); nuclei are stained in blue pseudocolor using DRAQ5. Western blot analysis of cancer cell lysates with Shc mAb (11F6); HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5), PC12 (6), CMT93 (7), Neuro 2A (8), NIH-3T3 (9). Immunohistochemistry analysis of human hepatoma tissue with Shc mAb (11F6).33419-42-0 manufacturer Immunofluorescent analysis (confocal) staining of V79 cells using Shc mAb (11F6) (green); nuclei are stained in blue pseudocolor using DRAQ5.2169919-21-3 custom synthesis Western blot analysis of cancer cell lysates with Shc mAb (11F6); HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5), PC12 (6), CMT93 (7), Neuro 2A (8), NIH-3T3 (9).PMID:30000240
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Additional Information :
| Alternative Name Src homology 2 domain containing | Application ELISA, ICC, IHC, WB | Application Notes Detects bands of ~46kDa, ~52kDa and ~66kDa by Western blot. | Clone 11F6 | Formulation Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. | Host Mouse | Immunogen Synthetic peptide corresponding to the sequence near the C-terminus of Shc. | Isotype IgG1 | Recommendation Dilutions/Conditions Immunohistochemistry (1:50)Western Blot (1µg/ml, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Reconstitution Reconstitute with 1ml water (15 minutes at room temperature). | Species Reactivity Human, Mouse | UniProt ID P29353 | Unit of Measure (UM) µg
