Product Name: EGFR (phospho Tyr845) antibody
Applications: ICC/IF, WB
Predicted Target Size:
Positive Controls: Human Epidermoid Carcinoma (A431) cells and cortic smooth muscle cells
Form Supplied: Liquid
Concentration:
Purification: Immunogen affinity purified
Full Name: epidermal growth factor receptor
Background: Epidermal Growth Factor Receptor, also known as ErbB 1 (EGFR, a 185 kDa glycoprotein) is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. The EGFR consists of a large extracellular domain, a single transmembrane domain and a cytoplasmic domain that exhibits kinase activity. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. These events result in EGFR activation and increased tyrosine kinase activity toward a variety of intracellular substrates. Tyrosine 845 a major autophosphorylation site in EGFR is mediated by Src, and regulates receptor function and tumor progression.
Synonyms: ERBB1, 131550, 1956, EGFR, ERBB, mENA, P00533, EGFR (phospho Y845), EGFR (phospho Tyr845), EGFR (pY845), EGFR (pTyr845), EGFR phospho Y845, EGFR phospho Tyr845, Phospho EGFR (Y845), Phospho EGFR (Tyr845), Phospho EGFR (pY845), Phospho EGFR (pTyr845)
Cellular Localization: Cell Membrane and Secreted
CAS NO: 711019-86-2
Product: MK-5108
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 845.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol 1mg/ml BSA. pH 7.3
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: The full-length EGFR includes the 24 aa signal peptide that is cleaved during EGFR maturation. The Tyr845 of mature EGFR is equivalent to Tyr869 of uncleaved EGFR.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23389444?dopt=Abstract
