Product Name: EGFR antibody [F4]
Applications: ELISA, IHC-P, IP, WB
Predicted Target Size:
Positive Controls:
Form Supplied: Liquid
Concentration:
Purification: Unpurified
Full Name: epidermal growth factor receptor
Background: The receptor for Epidermal Growth Factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. It is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. EGF-R has anextracellular region which binds EGF and consequently mediates the initial response of cells to EGF and an intra-cellular region which posseses the tyrosine kinase activity. As a result of EGF binding to its specific receptor, there is increased DNA synthesis as well as other events including cell proliferation, differentiation and repair of damaged epithelial tissue. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic and transmembrane part of the EGF-R (truncatedEGF-R). It is hypothesized that an inappropriate activation of the human erb-B gene either by truncation or overexpression plays a role in the development of the malignancy. This hypothesis is supported by studies, which have shown an increased number of EGF-R in various malignant tumors. High levels of EGF-R have been identified in sarcomas, gliomas, gynecological, breast, bladder, and lung tumors.
Synonyms: ERBB1, 131550, 1956, EGFR, ERBB, mENA, P00533
Cellular Localization:
CAS NO: 60133-18-8
Product: Nelfinavir
Host: Mouse
Clonality: Monoclonal
Isotype: IgG1
Immunogen: synthetically produced residues 985-996 of the human EGF receptor.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: Ascites, 15 mM sodium azide
Storage Instruction: Keep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This antibody recognizes specifically an intracellular domain of the EGF receptor.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24048590?dopt=Abstract
