Product Name: eIF2 alpha (phospho Ser52) antibody
Applications: ICC/IF, WB
Predicted Target Size:
Positive Controls: 3T3-L1 adipocytes stimulated with Leukemia Inhibitory Factor (LIF)
Form Supplied: Liquid
Concentration:
Purification: Immunogen affinity purified
Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Background: eIF-2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF-2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF-2 alpha is phosphorylated at serine 52 by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF-2 alpha blocks the GDP-GTP exchange activity of eIF-2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF-2 alpha is an important regulatory process in protein synthesis.
Synonyms: Eukaryotic Translation Initiation Factor2 alpha, EIF2A, EIF2A phospho S52, EIF-2A, P05198, EIF2S1, 603907, EIF2, EIF-2, 1965, Eukaryotic Translation Initiation Factor 2 alpha, EIF-2alpha, EIF2alpha, EIF 2, EIF 2A, EIF 2alpha, EIF2A (phospho S52), EIF2A (p
Cellular Localization:
CAS NO: 629664-81-9
Product: Geldanamycin
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic peptide (Human) derived from the region of human eIF-2 alpha that contains serine 52. This region is conserved among many species including rat, pig, cow, fruit fly, and yeast.
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23285215?dopt=Abstract
