Product Name: eIF4E (phospho Ser209) antibody
Applications: WB
Predicted Target Size: 25 kDa (note) (4)
Positive Controls: Treated HCT116 whole cell extracts , Treated HeLa whole cell extracts
Form Supplied: Liquid
Concentration: 1.68 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified by antigen-affinity chromatography.
Full Name: eukaryotic translation initiation factor 4E
Background: All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
Synonyms:
Cellular Localization:
CAS NO: 124937-52-6
Product: PI3K-IN-1
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human eIF4E (phospho Ser209). The exact sequence is proprietary.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21061049?dopt=Abstract
