Product Name: EndoG antibody [7F2G10]
Applications: ELISA, WB
Predicted Target Size:
Positive Controls: Lysates:Cat. No. GTX27900 – HepG2 Cell Lysate
Form Supplied: Liquid
Concentration:
Purification: Immunoaffinity chromotography purified IgG
Full Name: endonuclease G
Background: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Synonyms: EndoG (7F2G10): Endonuclease G
Cellular Localization:
CAS NO: 133550-30-8
Product: MRS 2578
Host: Mouse
Clonality: Monoclonal
Isotype:
Immunogen: Recombinant protein corresponding to amino acids 51 – 140 of human EndoG.
Antigen Species:
Species Reactivity: Human, Rat
Conjugation: Unconjugated
Storage Buffer: Antibody is supplied in PBS containing 0.02% sodium azide
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This antibody is specific for EndoG
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21921037?dopt=Abstract
