Product Name: Epac1 antibody
Applications: ELISA, WB
Predicted Target Size:
Positive Controls:
Form Supplied:
Concentration: 0.78 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Immunogen affinity purified
Full Name: Rap guanine nucleotide exchange factor (GEF) 3
Background: The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography.
Synonyms: EPAC, MGC21410, 606057, RAPGEF3, EPAC1, O95398, bcm910, CAMP-GEFI, CAMPGEFI, 10411, HSU79275, CAMP GEFI
Cellular Localization:
CAS NO: 923287-50-7
Product: LY2228820
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic peptide: PRSCSYQLLLEHQRPSCI, corresponding to amino acids 9-26 of Epac1.
Antigen Species: Human
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Storage Buffer:
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: GTX41235 does not cross react with Epac 2.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24736629?dopt=Abstract
