Product Name: ITCH antibody, Internal
Applications: WB
Predicted Target Size:
Positive Controls:
Form Supplied: Liquid
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Full Name: itchy E3 ubiquitin protein ligase homolog (mouse)
Background: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes Lys-29-, Lys-48- and Lys-63-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates Lys-63 polyubiquitin chains on RIPK1 and catalyzes the formation of Lys-48-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by Lys-63-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by Lys-63 linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through Lys-48-linked conjugation resulting in MAVS proteosomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteosomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteosomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through Lys-29-linked polyubiquitination.
Synonyms: ITCH / AIF4, NAPP1, atrophin1 interacting protein 4, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse), NFE2associated polypeptide1, NFE2-associated polypeptide 1, atrophin1 interacting protein4, ubiquitin protein ligase ITCH, dJ468O1.1 (atrophin 1 interacting, NFE2associated polypeptide 1, dJ468O1.1, dJ468O1.1 (atrophin1 interacting, AIP4, NFE2 associated polypeptide 1
Cellular Localization: Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1.
CAS NO: 1429881-91-3
Product: L-Cysteine methyl ester (hydrochloride)
Host: Goat
Clonality: Polyclonal
Isotype: IgG
Immunogen: C-EIKSHDLKPNGGN
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Instruction: Aliquot and store at -20°C. Minimize freezing and thawing.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This antibody is specific for ITCH.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22797921?dopt=Abstract
