Product Name: Lamin B1 antibody [119D5-F1]
Applications: ELISA, FACS, ICC/IF, IHC, IP, WB
Predicted Target Size: 67 kDa (note)
Positive Controls:
Form Supplied: Liquid
Concentration:
Purification:
Full Name: lamin B1
Background: An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A and B type lamins.
Synonyms: LMB1, LMN, LMN2, LMNB 1, LMNB, MGC111419,
Cellular Localization:
CAS NO: 2398-96-1
Product: ACTB-1003
Host: Mouse
Clonality: Monoclonal
Isotype: IgG1
Immunogen: Purified rat liver lamins
Antigen Species: Rat
Species Reactivity: Human, Mouse, Dog, Rat, Zebrafish, Rabbit, Sheep, Bovine, Sheep
Conjugation: Unconjugated
Storage Buffer: PBS containing 0.09% sodium azide
Storage Instruction: Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: Reacts with an epitope located C-terminal of residue 231 in lamin B1. Reacts with lamin B1 from human, rat, mouse, bovine, rabbit, dog, sheep
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22354151?dopt=Abstract
