Product Name: Laminin gamma 2 antibody [N3C1], Internal
Applications: IHC-P, WB
Predicted Target Size: 131 kDa (note) (3)
Positive Controls: Raji , NCI-H929
Form Supplied: Liquid
Concentration: 0.66 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified by antigen-affinity chromatography.
Full Name: laminin, gamma 2
Background: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3 terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq]
Synonyms: B2T antibody, BM600 antibody, CSF antibody, EBR2 antibody, EBR2A antibody, LAMB2T antibody, LAMNB2 antibody, MGC138491 antibody, MGC141938 antibody, LAMC2 antibody, nicein subunit gamma antibody, kalinin subunit gamma antibody, CSF 140 kDa subunit antibody, BM600-100kDa antibody, laminin B2t chain antibody, laminin subunit gamma-2 antibody, large adhesive scatter factor 140 kDa subunit antibody, epiligrin subunit gamma antibody, cell-scattering factor 140 kDa subunit antibody, ladsin 140 kDa subunit antibody, laminin, gamma 2 antibody
Cellular Localization: Secreted , extracellular space , extracellular matrix , basement membrane
CAS NO: 107-35-7
Product: XL-647
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Recombinant protein encompassing a sequence within the center region of human Laminin gamma 2. The exact sequence is proprietary.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27456084?dopt=Abstract
