Product Name: LATS1/2 antibody
Applications: ELISA, IHC-P
Predicted Target Size: 126 kDa (note)
Positive Controls:
Form Supplied:
Concentration:
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Full Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
Background: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.
Synonyms: Serine/threonine-protein kinase k, Serine/threonineprotein kinase kpm, hwarts/EC 2.7.11.1, LATS1/2, Wartslike kinase, Large tumor suppressor homolog 2, Large tumor suppressor homolog 1, WARTS protein kinase, Warts-like kinase, Large tumor suppressor homolog1, h-warts/EC 2.7.11.1, Large tumor suppressor homolog2, hwarts/EC2.7.11.1, Serine/threonineprotein kinase k, Warts like kinase, h warts/EC 2.7.11.1
Cellular Localization: Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
CAS NO: 34597-40-5
Product: LDN-214117
Host: Rabbit
Clonality: Polyclonal
Isotype:
Immunogen: The antiserum was produced against synthesized peptide derived from human LATS1/2.
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction: Store at -20 degrees Celcius. Product is stable for at least 1 year.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: LATS1/2 (Ab-1079/1041) Antibody detects endogenous levels of total LATS1/2 protein.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18321952?dopt=Abstract
