Product Name: LIME antibody [LIME-06]
Applications: IP, WB
Predicted Target Size:
Positive Controls:
Form Supplied:
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Protein A purified
Full Name:
Background:
Synonyms:
Cellular Localization:
CAS NO: 364622-82-2
Product: Pixantrone (dimaleate)
Host: Mouse
Clonality: Monoclonal
Isotype: IgG1
Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Antigen Species:
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25634525?dopt=Abstract
