Product Name: LIME antibody [mLIME-05]
Applications: IP, WB
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Full Name: Lck interacting transmembrane adaptor 1
Background: LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Entrez Gene: 2 H4
Synonyms:
Cellular Localization: Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell.
CAS NO: 79559-97-0
Product: BX471 (hydrochloride)
Host: Mouse
Clonality: Monoclonal
Isotype: IgG2a
Immunogen: Recombinant protein corresponding to amino acids 86-202 of mouse LIME
Antigen Species: Mouse
Species Reactivity: Mouse
Conjugation: Unconjugated
Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Instruction: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26193221?dopt=Abstract
