Product Name: DNMT1 antibody [60B1220.1]
Applications: IHC-P, IP, WB, ChIP assay
Predicted Target Size:
Positive Controls:
Form Supplied: Liquid
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration)
Purification: protein G purified
Full Name: DNA (cytosine-5-)-methyltransferase 1
Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Synonyms: DNA (cytosine5 ) methyltransferase1 antibod, DNA methyltransferase1, FLJ16293, MGC104992 Antibody, DNA methyltransferase M.HsaI., DNMT, DNMT1, CXXC9, DNA MTase, M.HsaI, MCMT, DNA (cytosine 5 ) methyltransferase 1 antibod, DNMT 1, DNA MTase HsaI, DNA methyltransferase HsaI, DNA methyltransferase 1
Cellular Localization:
CAS NO: 132836-42-1
Product: (+)-MK 801 (Maleate)
Host: Mouse
Clonality: Monoclonal
Isotype: IgG1 Kappa
Immunogen: This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
Antigen Species: Human
Species Reactivity: Human, Mouse, Zebrafish
Conjugation: Unconjugated
Storage Buffer: PBS containing 0.2% gelatin
Storage Instruction: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This can be used in immunohistochemistry at 1-2 ug/ml in human kidney cells.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21885567?dopt=Abstract
