Product Name: DNMT3A antibody [64B814]
Applications: IHC, WB
Predicted Target Size:
Positive Controls: 293 cell lysate
Form Supplied: Liquid
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Protein G purified
Full Name: DNA methyltransferase 3A
Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt 1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Synonyms: W91664, 1788, DNMT3A2, M.HsaIIIA, Q9Y6K1, Dnmt3a, 602769, 13435, AU015806
Cellular Localization: Nuclear
CAS NO: 550999-74-1
Product: Toremifene (Citrate)
Host: Mouse
Clonality: Monoclonal
Isotype: IgG1
Immunogen: Bacteria expressed HIS-tag recombinant mouse Dnmt3a.
Antigen Species: Mouse
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: Preservative: 0.02% Sodium Azide; Constituents: PBS
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21773054?dopt=Abstract
