Product Name: ERK1/2 (phospho Thr185/Tyr187) antibody
Applications: WB
Predicted Target Size:
Positive Controls: PC12 cells +/- Sorbitol
Form Supplied: Liquid
Concentration:
Purification: Immunogen affinity purified
Full Name: mitogen activated protein kinase 1
Background: ERK (extracellular signal-regulated kinase), also known as MAPK (mitogen-activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signalling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK 1 and ERK 2 are fully activated by phosphorylation within a Thr-Glu-Tyr motif of the activation loop on both a threonine and a tyrosine residue by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of the ERK 1 + 2 enzymes.
Synonyms: ERK1 & ERK2, 176982, PKC2, 116590, Erk2, 5590, Q05513, Mapk1, PRKCZ, ERK1 (phospho T185/T202), ERK1 (phospho Thr185/Thr202), ERK1 (pT185/pT202), ERK1 (pThr185/pThr202), ERK1 phospho T185/T202, ERK1 phospho Thr185/Thr202, Phospho ERK1 (T185/T202), Phospho ERK1 (Thr185/Thr202), Phospho ERK1 (pT185/pT202), Phospho ERK1 (pThr185/pThr202)
Cellular Localization:
CAS NO: 193153-04-7
Rucaparib (phosphate)
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic peptide (Human) derived from the region of ERK 1 + 2 that contains threonine 202/185 and tyrosine 204/187, based on the human sequence. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: The sequence of the immunogenic peptide used to generate GTX24819 includes the amino acids found in the region of both ERK1 and ERK2 including specific threonine and tyrosine residues. In ERK1, these residues are at positions 185 and 187, respectively. In ERK2, these same residues are actually at positions 202 and 204, respectively of the native protein. The antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24632884?dopt=Abstract
