Product Name: E-Cadherin antibody [DECMA-1]
Applications: ICC/IF, IHC-Fr, IP, WB
Predicted Target Size:
Positive Controls: MDCK
Form Supplied:
Concentration:
Purification: Unpurified
Full Name: cadherin 1
Background: Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca2+.
Synonyms: E-cadherin, Arc-1, CDHE, AA960649, 12550, Cdh1, 137215, Um, P12830, Ecadherin, Ecad, UVO, 999, uvomorulin, MGC107495, E Cadherin, LCAM, Arc1, Arc 1
Cellular Localization:
CAS NO: 957054-33-0
Product: SCH 563705
Host: Rat
Clonality: Monoclonal
Isotype: IgG1
Immunogen: mouse embryonal carcinoma cell line PCC4 Aza R1.
Antigen Species: Mouse
Species Reactivity: Human, Mouse, Dog, Bovine
Conjugation: Unconjugated
Storage Buffer: Ascites, 15 mM sodium azide
Storage Instruction: Keep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: Monoclonal Anti-Uvomorulin/E-Cadherin was selected against the mouse cell adhesion molecule uvomorulin/E-Cadherin. The antibody localizes the cell surface glycoprotein uvomorulin/E-cadherin that has been found to be identical to L-CAM, Cell CAM 80/120, and ARC-1. It blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. The antibody disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. In indirect immunofluorescent staining of MDCK cells grown in culture, the antibody shows strong staining on the membrane of adjacent cells, after treatment with 0.5% Triton-X 100.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/24646567?dopt=Abstract
