Product Name: EndoG antibody
Applications: IHC-P, WB
Predicted Target Size:
Positive Controls: HepG2 cell lysate
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Immunogen affinity purified
Full Name: endonuclease G
Background: The activities of caspase and nuclease are involved in DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Studies have demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Synonyms: Endonuclease G, 600440, Q14249, 2021, ENDOG
Cellular Localization: Mitochondrial
CAS NO: 803712-79-0
Product: WAY-100635
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic peptide: GGPRGPGELAKYGLP, corresponding to amino acids 55-70 of Human Endonuclease G.
Antigen Species: Human
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Storage Buffer: Preservative: 0.02% Sodium Azide; Constituents: PBS
Storage Instruction: Upon receipt – Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This antibody recognizes human, mouse, and rat EndoG (Endonuclease G).
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21784972?dopt=Abstract
