Product Name: Ki67 antibody [PP-67]
Applications: ICC/IF, IHC-P
Predicted Target Size:
Positive Controls: human tonsil
Form Supplied: Liquid
Concentration:
Purification: Unpurified
Full Name: marker of proliferation Ki-67
Background: Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong.
Synonyms: KIA
Cellular Localization:
CAS NO: 979-32-8
Product: Piboserod (hydrochloride)
Host: Mouse
Clonality: Monoclonal
Isotype: IgM
Immunogen: HeLa cell nuclear preparation.
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: Ascites, 15 mM sodium azide
Storage Instruction: Keep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: Reacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower m.w. proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. It labels proliferating cells in frozen human tissue and in formalin-fixed, paraffin-embedded tissue. A microwave heating retrieval step is required for successful labeling of routinely formalin-fixed, paraffin-embedded human tissue.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21984751?dopt=Abstract
