Product Name: Kinesin antibody [KN-02]
Applications: ICC/IF
Predicted Target Size:
Positive Controls:
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified by thiophilic adsorption-affinity chromatography
Full Name:
Background: Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Synonyms: kinesin 1 (110-120kD) kinesin family member 5B Kinesin heavy chain ubiquitous UKHC
Cellular Localization: Microtubules
CAS NO: 81409-90-7
Product: RBC8
Host: Mouse
Clonality: Monoclonal
Isotype: IgM
Immunogen: Enriched fraction of porcine brain kinesin.
Antigen Species:
Species Reactivity: Human, Mouse, Pig, Rat
Conjugation: Unconjugated
Storage Buffer: Phosphate buffered saline pH 7.4 containing 15mM sodium azide
Storage Instruction:
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: The antibody reacts with heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. The antibody does not react with kinesin bound to taxol-stabilized microtubules.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19539741?dopt=Abstract
