Product Name: Ku80 antibody, Internal
Applications: WB
Predicted Target Size:
Positive Controls: The peptide used to product this antibody is available for purchase (Catalog Number:GTX89363-PEP).
Form Supplied: Liquid
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Full Name: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Background: Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3-5 direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5-deoxyribose-5-phosphate lyase (5-dRP lyase), by catalyzing the beta-elimination of the 5 deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5-dRP activity, and allows to clean the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined.
Synonyms: Xray repair, complementing defective, repair in Chinese hamster, Xray repair complementing defective repai, Ku86, ATPdependent DNA helicase II, NFIV, DNA repair protein XRCC5, ATP-dependent DNA helicase II, Ku80 / XRCC5, X-ray repair complementing defective repai, HGNC:12833, XRCC5, KU80, FLJ39089, KARP-1, KARP1, KUB2, KARP 1, ATP dependent DNA helicase II
Cellular Localization:
CAS NO: 141505-33-1
Product: Bephenium (hydroxynaphthoate)
Host: Goat
Clonality: Polyclonal
Isotype: IgG
Immunogen: C-SLAKKDEKTDTLED
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Instruction: Aliquot and store at -20°C. Minimize freezing and thawing.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This antibody is specific for Ku80 / XRCC5.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/23917429?dopt=Abstract
