Product Name: LEF1 antibody, Internal
Applications: WB
Predicted Target Size:
Positive Controls: The peptide used to product this antibody is available for purchase (Catalog Number:GTX89284-PEP).
Form Supplied: Liquid
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Full Name: lymphoid enhancer-binding factor 1
Background: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Synonyms: lymphoid enhancerbinding factor 1, lymphoid enhancer-binding factor 1, DKFZp586H0919, LEF1, lymphoid enhancer binding factor1, TCF1ALPHA, lymphoid enhancerbinding factor1, lymphoid enhancer binding factor-1, HGNC:6551, FLJ46390
Cellular Localization: Found in nuclear bodies upon PIASG binding.
CAS NO: 36322-90-4
Product: Exherin
Host: Goat
Clonality: Polyclonal
Isotype: IgG
Immunogen: C-QHEQRKEQEPKRPH
Antigen Species: Human
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Storage Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Instruction: Aliquot and store at -20°C. Minimize freezing and thawing.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: This antibody is specific for LEF1.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/18248364?dopt=Abstract
