Product Name: LIME antibody [LIME-06] (PE)
Applications: IP, WB
Predicted Target Size:
Positive Controls:
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Full Name: Lck interacting transmembrane adaptor 1
Background: LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals. Entrez Gene: 20q13.3
Synonyms: FLJ20406, LIME, LP8067, dJ583P15.4
Cellular Localization: Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell.
CAS NO: 148016-81-3
Product: WAY-262611
Host: Mouse
Clonality: Monoclonal
Isotype: IgG1
Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Phycoerythrin (PE)
Storage Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Storage Instruction: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is sto
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25685069?dopt=Abstract
