Product Name: LIM kinase 2 antibody
Applications: ICC/IF, IHC-P, WB
Predicted Target Size: 72 kDa (note) (3)
Positive Controls: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified by antigen-affinity chromatography.
Full Name: LIM domain kinase 2
Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Synonyms: LIMK2 antibody, LIM domain kinase 2 antibody
Cellular Localization: Isoform LIMK2a: Cytoplasm , Nucleus , Isoform LIMK2b: Cytoplasm
CAS NO: 19216-56-9
Product: Amicarbazone
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Recombinant protein encompassing a sequence within the center region of human LIM kinase 2. The exact sequence is proprietary.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26732624?dopt=Abstract
