Product Name: Lck antibody [33D196]
Applications: WB
Predicted Target Size:
Positive Controls: Jurkat cell lysate.
Form Supplied:
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Protein G purified
Full Name: lymphocyte-specific protein tyrosine kinase
Background: Lck is a lymphoid-specific cytosolic protein tyrosine kinase (PTK), which is essential for T-cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAMs) in the cytoplasmic tails of the TCR-chains and CD3 subunits. The phospho-ITAMs serve as docking sites for Src homology domain 2 (SH2)-containing molecules, predominantly ZAP-70 and Syk. Only then can ZAP-70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.
Synonyms: LSK, YT16, p56lck, pp58lck
Cellular Localization: Cytoplasmic
CAS NO: 179411-93-9
Product: DZ2002
Host: Mouse
Clonality: Monoclonal
Isotype: IgG2b
Immunogen: Synthetic peptide (Human).
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: Preservative: 0.02% Sodium Azide; Constituents: PBS.
Storage Instruction: Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19049981?dopt=Abstract
