Product Name: Lin28A antibody [GT1086]
Applications: FACS, ICC/IF, IHC-P, WB
Predicted Target Size: 23 kDa (note) (2)
Positive Controls: NT2D1 , human ESC , LIN28 transfected HeLa lysates
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Affinity purified by Protein G.
Full Name: lin-28 homolog A (C. elegans)
Background: Acts as a translational enhancer, driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5-GGAG-3 motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5-GGAG-3 motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Synonyms: CSDD1 antibody, FLJ12457 antibody, LIN-28 antibody, LIN28 antibody, ZCCHC1 antibody, LIN28A antibody, zinc finger CCHC domain-containing protein 1 antibody, protein lin-28 homolog A antibody, zinc finger, CCHC domain containing 1 antibody, RNA-binding protein LIN-28 antibody, lin-28 homolog A (C. elegans) antibody
Cellular Localization: Cytoplasm , Nucleus , nucleolus
CAS NO: 896106-16-4
Product: Dictamine
Host: Mouse
Clonality: Monoclonal
Isotype: IgG2b
Immunogen: Recombinant protein encompassing a sequence within the center region of human LIN28. The exact sequence is proprietary.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: PBS
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/25180064?dopt=Abstract
