Product Name: eIF2 alpha (phospho Ser49) antibody
Applications: ICC/IF, IHC-P, WB
Predicted Target Size: 38 (note)
Positive Controls:
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Background: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Synonyms: EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, EIF2S1, eukaryotic translation initiation factor 2 subunit 1, eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Cellular Localization:
CAS NO: 284035-33-2
Product: PSI-6206
Host: Rabbit
Clonality: Polyclonal
Isotype:
Immunogen: Peptide sequence around phosphorylation site of Serine 49 (L-L-S(p)-E-L) derived from Human eIF2 alpha .
Antigen Species: Human
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Storage Buffer: saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22916109?dopt=Abstract
