Product Name: eIF4G (phospho Ser1232) antibody
Applications: IHC-P, WB
Predicted Target Size: 220 (note)
Positive Controls:
Form Supplied: Liquid
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Full Name: eukaryotic translation initiation factor 4 gamma, 1
Background: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
Synonyms: DKFZp686A1451, EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, EIF4G1, eucaryotic translation initiation factor 4G, EIF4-gamma, eukaryotic translation initiation factor 4 gamma 1, eIF-4-gamma 1, eukaryotic translation initiation factor 4 gamma, 1
Cellular Localization:
CAS NO: 957116-20-0
Product: GSK1059615
Host: Rabbit
Clonality: Polyclonal
Isotype:
Immunogen: Peptide sequence around phosphorylation site of serine 1232 (P-V-S(p)-P-L) derived from Human eIF4G.
Antigen Species: Human
Species Reactivity: Human
Conjugation: Unconjugated
Storage Buffer: saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20504962?dopt=Abstract
