Product Name: eRF1 antibody
Applications: ICC/IF, IHC-P, WB
Predicted Target Size: 49 kDa (note)
Positive Controls: Raji
Form Supplied: Liquid
Concentration: 0.8 mg/ml (Please refer to the vial label for the specific concentration)
Purification: Purified by antigen-affinity chromatography.
Full Name: eukaryotic translation termination factor 1
Background: Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]
Synonyms: D5S1995 antibody, ERF antibody, ERF1 antibody, MGC111066 antibody, RF1 antibody, SUP45L1 antibody, TB3-1 antibody, ETF1 antibody, eukaryotic release factor 1 antibody, eukaryotic peptide chain release factor subunit 1 antibody, polypeptide chain release factor 1 antibody, protein Cl1 antibody, sup45 (yeast omnipotent suppressor 45) homolog-like 1 antibody, eukaryotic translation termination factor 1 antibody
Cellular Localization: Cytoplasm
CAS NO: 357-70-0
Navitoclax
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human eRF1. The exact sequence is proprietary.
Antigen Species: Human
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction: Keep as concentrated solution. Aliquot and store at -20ÂșC or below. Avoid multiple freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Specificity:
PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27660242?dopt=Abstract
