Worth was proven S the S treatment, was amended with the digestate containing a higher S-SO4 2- D-Lysine monohydrochloride In Vivo concentration (Table 5). five). ARS moderately correlated PHOS (r = 0.58) which a higher S-SO42- concentration (Table ARS moderately correlated toto PHOS (r =0.58) which was statistically the highest in the therapy and lowest within the BC (Figure 2c). The last was statistically the highest within the S S treatment and lowest within the BC (Figure 2c). The final determined enzyme was in comparison towards the the handle significantly increased in determined enzyme UREURE was in comparison tocontrol considerably increased in sulsulphur amended remedies + S and S (Figure 2d). phur amended treatments BCBC + S and S (Figure 2d).Figure 2. Soil activities of – glucosidase–GLU (a), arylsulfatase–ARS (b), phosphatase–PHOS (c),(c), and urease–URE Figure 2. Soil activities of – glucosidase–GLU (a), arylsulfatase–ARS (b), phosphatase–PHOS and urease–URE (d); (d); tested remedies: BC–biochar, S–sulphur, + S–biochar and and sulphur. Imply SD. The distinctive letters express tested treatments: BC–biochar, S–sulphur, BC BC + S–biochar sulphur. Mean SD. The distinct letters express the the outcomes of ANOVA Tukey’s HSD Posthoc Test–the Gardiquimod In Vitro statistical differences at significance level0.05.0.05. benefits of ANOVA Tukey’s HSD Posthoc Test–the statistical variations at significance level p pThe values of BR within the BC and S S treatments were substantially decrease comparedthe The values of BR within the BC and treatments were significantly lower compared to for the control (Figure 3a), showing that aerobic decomposition is apparently negatively afcontrol (Figure 3a), displaying that aerobic decomposition is apparently negatively affected fected by the amendment respective enriched digestates. The co-enrichment of digestate by the amendment of the from the respective enriched digestates. The co-enrichment of digestate with both the biochar and elemental sulphur mitigates the negative of each on the with both the biochar and elemental sulphur mitigates the damaging impact effect of each in the materials on the the within the soil. supplies on the BR in BR soil. As all SIRs correlated highly or moderately positively with every single other, the variations all SIRs correlated extremely or moderately positively with each and every other, the variations within the respiration properties have been comparable (Figure 3b ). One example is, the BC and S treatrespiration properties were comparable (Figure 3b ). For instance, the BC and S treatments’ values were significantly reduce than the manage. In contrast, the BC + S digestate ments’ values drastically elevated or didn’t transform all SIRs and we assumed that the combined enrichment of improved digestate by biochar and sulphur mitigated the adverse impact of either BC or elemental Son by biochar and sulphur mitigated the adverse effect of either BC or elemental soil soil aerobes. Furthermore, the PCA (Figure A2) showed a optimistic connection amongst Son aerobes. Furthermore, the PCA biplotbiplot (Figure A2) showed a positive partnership all sorts of soil of soil respiration except for Glc-SIR. amongst all typesrespiration except for Glc-SIR.Agronomy 2021, 11, 2041 Agronomy 2021, 11, x FOR PEER REVIEW8 of 14 eight ofFigure three. Basal respiration trehalose SIR–Tre-SIR (b), (b), L-lysine SIR–Lys-SIR (c), L-alanine Figure 3. Basal respiration (a),(a), trehalose SIR–Tre-SIR L-lysine SIR–Lys-SIR (c), L-alanine SIR– SIR–Ala-SIR (d), D-glucose SIR–Glc-SIR (e) and N-acetyl–D-glucosamine SIR.