Usion: Medin and MFGE8 are abundant in aged subjects and are secreted by exosomes in to the ECM. Exosome release is increased with age, which could contribute for the deposition of medin in the ECM along with the formation of amyloid. MFGE8 might play a function in accelerating calcification by inducing an osteogenic phenotype through the ERK pathway. Each MFGE8 and medin secretion by exosomes could contribute for the age-related development of vascular calcification. Funding: This work is funded by the British Heart Foundation.utilized as cellular ageing model. Dx accelerated ageing, but Wnt4-containing exosomes could efficiently counteract Dx-induced senescence. We have obtained diverse staining patterns using DiI-labelled Wn4-exosomes on sections of young and aged samples. Ultimately, in vivo injected DiI-labelled Wnt4-exosomes ETB Antagonist Species showed detectable homing to the thymus. Summary/Conclusion: In accordance with our results Wnt4 and miR27b are present in TEC exosomes. Our findings indicate that Wnt4 is often a key inhibitor D2 Receptor Inhibitor manufacturer thymic involution potentially via miR27b. Nevertheless, further experiments are necessary for possible applications. Funding: Scientific analysis assistance was supplied by PTE AOK KA2016-16, PTE Pharmaceutical Talent Center program as well as the PTE Viral Pathogenesis Talent Center program via KK. The Janos Bolyai Scholarship in the Hungarian Academy of Sciences also supported KK.PS06.Extracellular vesicles and their miRNA cargo in ageing and ageassociated illnesses Lucia Terlecki-Zaniewicz1; Vera Pils1; Ingo L mermann1; Regina Weinm lner1; Madhusudan Bobbili Reddy1; Markus Schosserer1; Florian Gruber2; Matthias Hackl3; Johannes Grillari1 CDL for Biotechnology of Skin Aging BOKU Department of Biotechnology, Vienna, Austria; 2Department of Dermatology, Medical University of Vienna, Austria; Christian Doppler Laboratory for the Biotechnology of Skin Aging, Vienna, Austria, Vienna, Austria; 3TAmiRNA GmbH Vienna, Vienna, AustriaPS06.11 = OWP1.Role of Wnt4 exosomes in thymic ageing Krisztina Banfai1; Kitti Garai1; David Ernszt2; Judit E. Pongracz1; Krisztian KvellInstitute of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Pecs, Hungary, P s, Hungary; 2Institute of Physiology, Faculty of Medicine, University of Pecs, Pecs, Hungary, P s, HungaryBackground: Wnt4 plays a critical role in advertising the improvement and halting the ageing from the thymus. Through ageing Wnt4 is downregulated, whilst PPAR is up-regulated and triggers adipose involution. Having said that, miR27b was described to suppress PPAR. Our goal was to prove the presence of Wnt4 in exosomes, to detect its impact and adhere to its path both in vitro and in vivo. Methods: Exosomes had been harvested from manage and Wnt4 overexpressing TECs (thymic epithelial cells) for additional experiments. Exosomes were visualized by transmission electron microscopy. Exosomal miR27b levels have been measured by TaqMan qPCR, though Wnt4 protein content material was assayed by ELISA. DiI-labelled exosomes were applied on mouse and human thymus sections and also iv-injected into mouse for in vivo tracking. Outcomes: Transmission electron microscopy showed exosomes ranging 50100 nm in size. TaqMan miRNA assay measured elevated miR27b levels, when ELISA showed higher Wnt4-content in Wnt4-exosomes when compared with control exosomes. For functional studies steroid (Dx)-induced TECs wereBackground: Cellular senescence has evolved from an in vitro model program to study ageing to a multifaceted phenomenon of in vivo value as senescent cell removal delays t.
