High expression levels inside the parathyroid gland, stomach, pancreas, kidneys, epididymis, and prostate. The HPA database also showed that the parathyroid gland, gastrointestinal tract (stomach, duodenum, little intestine, and rectum), kidney, pancreas, and male tissues (epididymis, prostate, and seminal vesicle) had higher expression levels of both TMPRSS2 gene and protein. Earlier studies have shown that ACE2 has high expression levels in male tissues (testis and seminal vesicle) . Taken together, these information indicate that SARS-CoV-2 infection may perhaps affect male reproductive functions. 3.two. Correlations in between TMPRSS2 expression and immune signatures Within the pituitary, esophagus, colon, pancreas, breast, brain, skin, HDAC8 Inhibitor review salivary gland, and thyroid, TMPRSS2 expression levels had been positively correlated with all the Kainate Receptor Agonist Synonyms enrichment levels of B cells (FDR 0.05, 0.18 r 0.91) in each males and females (Fig. 2A). In the stomach and blood vessel, the significant correlation among TMPRSS2 expression levels and B cell enrichment levels had been only observed in females (0.ten r 0.19). Similarly, in the smaller intestine, stomach, esophagus, breast, brain, blood vessel, salivary gland, skin, and colon, CD8+ T cell enrichment levels had good correlations with TMPRSS2 expression levels in each males and females (0.06 r 0.73) (Fig. 2A). Nevertheless, TMPRSS2 expression showed substantial constructive correlations together with the CD8+ T cell signature solely inside the male bladder (r = 0.33) and pancreas (r = 0.88). Within the esophagus, bladder, colon, stomach, blood vessel, salivary gland, and skin, TMPRSS2 showed good expression correlations using the enrichment levels of NK cells in both males and females (0.30 r 0.80). In addition to, TMPRSS2 expression was positively correlated with the NK cell signature inside the female blood vessel (r = 0.13), male breast (r = 0.32), and male brain (r = 0.36). However, within the lungs and liver, TMPRSS2 expression levels were negatively correlated withNK cell enrichment levels in each males and females ( 0.30 r 0.17). For the interferon response signature, TMPRSS2 showed positive correlations with it in the pancreas, breast, brain, and skin (0.11 r 0.58), whilst negative correlations within the lungs, colon, esophagus, stomach, and little intestine ( 0.54 r 0.15) in each males and females. We further analyzed the correlations in between TMPRSS2 expression and immune signatures in younger and older cohorts, respectively (Fig. 2B). For B cells, CD8+ T cells, and NK cells, their enrichment levels were most likely to be positively correlated with TMPRSS2 expression levels in individual tissues in both younger and older cohorts. On the other hand, the enrichment levels of NK cells had been negatively correlated with TMPRSS2 expression levels in the liver, lungs, and thyroid in both younger and older cohorts ( 0.32 r 0.14). For the interferon response signature, TMPRSS2 showed constructive correlations with it within the pancreas and skin (0.32 r 0.55) and adverse correlations in the colon, esophagus, lungs, vagina, little intestine, and stomach ( 0.57 r 0.12). three.3. Pathways and GO associated with TMPRSS2 expression Using the gene set enrichment evaluation tool GSEA , we identified several pathways extremely enriched in the high-TMPRSS2-expression-level pan-tissue. These pathways had been primarily linked with immune, cell growth and proliferation, cancer and also other ailments, metabolism, and stromal signatures (Fig. 3A). The immune-related pathways incorporated the complement and co.