NUCLEAR-ID® Red DNA stain

Description:
Cell permeable DNA stain that can be used for a wide range of applications Far-red fluorescent specific DNA dye Stable and highly pure Live, permeable and fixed cells can be analyzed No photobleaching effect No RNase treatment is required GFP and FITC compatible UV laser source is not required for excitation Validated for a wide range of cell densitites Quick and easy to use! The NUCLEAR-ID® Red DNA Stain is a cell permeable dye, designed for use in a range of fluorescence detection technologies, in the discrimination of nucleated cells. It is resistant to photobleaching and is suitable for live-cell staining of nuclei. Also this dye provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry. Potential applications of this reagent for live-cell studies are in the determination of cellular DNA content and cell cycle distribution, for the detection of variations in growth patterns, for monitoring apoptosis, and for evaluating tumor cell behavior and suppressor gene mechanisms. NUCLEAR-ID® Red DNA stain spectra NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or a competitor’s dye at the equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min. NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or with a competitor’s dye at an equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min and 24h. Results show that 4000x NUCLEAR-ID Red DNA Stain was required for visualization of the dsDNA, while equivalent to 2000x was required for the competitor’s dye. At 24h, the competitor’s dye intensity and cell growth were dramatically reduced at the 2000x equivalent final concentration. At the same time point, 2000x of NUCLEAR-ID Red DNA Stain did not affect cell growth or fluorescent intensity. The NUCLEAR-ID Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs. Relative costs of using NUCLEAR-ID Red DNA in comparison to competitor dye in various applications: (A) Imaging (visualization), (B) Nucleated Cell Gating (flow cytometry) and (C) Live Cell Cycle analysis using flow cytometry. Dilutions can vary depending on cell strain and cell concentration.Notes:Assumes staining of a 100 µL staining volumeAssumes staining of a 500 µL cell suspension volumeAssumes a staining of 500 µL cell suspension volume NUCLEAR-ID® Red DNA stain spectra NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or a competitor’s dye at the equivalent µM concentration at 37°C and gently washed post-staining. Cells were imaged at 15 min. NUCLEAR-ID Red DNA Stain requires lower concentration than competitor’s dye to visualize dsDNA.HeLa cells were grown to ~60% confluency. Cells were stained with NUCLEAR-ID Red DNA Stain at a final concentration of 4000x or 2000x or with a competitor’s dye at an equivalent µM concentration at 37°C and gently washed post-staining.{{218136-59-5} web|{218136-59-5} Biological Activity|{218136-59-5} Formula|{218136-59-5} custom synthesis} Cells were imaged at 15 min and 24h.{{2170136-65-7} web|{2170136-65-7} Technical Information|{2170136-65-7} In stock|{2170136-65-7} custom synthesis} Results show that 4000x NUCLEAR-ID Red DNA Stain was required for visualization of the dsDNA, while equivalent to 2000x was required for the competitor’s dye. At 24h, the competitor’s dye intensity and cell growth were dramatically reduced at the 2000x equivalent final concentration.PMID:29489278 At the same time point, 2000x of NUCLEAR-ID Red DNA Stain did not affect cell growth or fluorescent intensity. The NUCLEAR-ID Red DNA Stain shows lower cytotoxicity and requires lower concentration in live cell studies, resulting in lower costs. Relative costs of using NUCLEAR-ID Red DNA in comparison to competitor dye in various applications: (A) Imaging (visualization), (B) Nucleated Cell Gating (flow cytometry) and (C) Live Cell Cycle analysis using flow cytometry. Dilutions can vary depending on cell strain and cell concentration.Notes:Assumes staining of a 100 µL staining volumeAssumes staining of a 500 µL cell suspension volumeAssumes a staining of 500 µL cell suspension volume