The effect of initial conditions and RANKL treatment on long-term osteoclast dynamics We next assessed the effects of the plating density and RANKL
.6% of the contigs down-regulated at fc #0.5 and 10.3% up-regulated at fc $2....
GRP78/BiP also favors cell survival and contributes to tumor progression and drug resistance during ER stress that arises in the tumor microenvironment
ncubated with secondary antibody at 37uC for 30 min. Immunohistochemical staining was visualized by...
Whereas in some experiments only a single peak of osteoclast formation was observed, in other experiments several waves of osteoclast formation and death were evident
e considerations led us to thoroughly characterize the homogeneous model of complete transection of...
a number of peptides and antibodies binding to the ATPase and substrate binding domains of GRP78/BiP have been reported. Some of these affect the growth promoting and angiogenic action of GRP78/BiP positively or negatively but their modes of action have not been extensively investigated
Mitochondria prepared with the protease meth- Experiments on isolated cardiomyocytes Cardiomyocyte isolation. Ventricular cardiomyocytes...
we adopted a refolding assay used previously to determine the chaperone activity of Hsp70 in vivo In this assay
ing the left ventricle, up-regulating the number and function of EPCs and increasing therapeutic...
The authors also thank Dr. Kirk Czymmek and the faculty, Bioimaging center, Delaware Biotechnology Institute for their help with microscopic studies
the endocardium, the thrombin expression levels were higher in the LA compared to the...
The bgalactosidase units produced per minute were estimated using the following protocol. Briefly, about 300 ml of the culture drawn at each time point were centrifuged at 14000 rpm at 4uC for 20 min
ses GR-mediated chromatin remodeling and transcription initiation and that methylation and acetylation at histone...
Monolayers were then washed in maleate buffer, incubated with uranyl acetate, washed again in maleate buffer, dehydrated in ascending alcohol washes
s research, our result showed that the men generally have been developing GC twice...
The DDB2 cDNA was also subcloned into a pEF1/Myc-HisB vector between the KpnI and XbaI sites, to produce a Myc-polyhistidine-tagged DDB2 protein
Muscle sequencing, a high-throughput transcriptomic approach, to provide an in-depth view of the transcriptome...
Five of the eight ERpositive samples showed a higher relative DDB2 mRNA level than the mean value, whereas one sample did not express DDB2
ons of WRN could be an important mechanism for the regulation of WRN functions...